Laboratory Research Scientist – Briscoe Lab

Job title: Laboratory Research Scientist – Briscoe Lab

Company: Francis Crick Institute

Job description: Salary for this Role:From £31,675 with benefits, subject to skills and experienceJob Title: Laboratory Research Scientist – Briscoe LabReports to: James BriscoeClosing Date: 09/Oct/2024 23.59 GMTJob Description:Laboratory Research Scientist – Briscoe LabReports to: Group LeaderThis is a full-time, fixed term (1 year) position on Crick terms and conditions of employment.BackgroundA Laboratory Research Scientist position is available in the Briscoe lab. The lab aims to understand the molecular and cellular mechanisms that underpin development, using the vertebrate neural tube as a model system. In this system, networks of transcription factors are responsible for interpreting signalling inputs and inducing the correct transcriptional response to ensure that the right cell forms in the right place and time. Understanding the molecular basis of these transcriptional networks will not only be vital for a proper understanding of development but will also pave the way for engineering cell behaviour for stem cell therapies.Given the complexity of this system, a major focus in the lab is the development of novel tools and assays that can describe transcriptional dynamics with greater resolution and throughput. In particular, the lab has recently implemented a protocol for single-cell transcriptomics through a method known as combinatorial indexing (detailed in Maizels RJ, Snell DM, Briscoe J. Reconstructing developmental trajectories using latent dynamical systems and time-resolved transcriptomics. (2024) Cell Systems 15:411-424).This method allowed them to analyse the transcriptome of single cells, tens of thousands of cells at a time. By combining this sequencing method with labelling of nascent RNA, they could model the dynamics of gene expression for in vitro differentiating neurons, providing greater insight into the dynamics of cell fate decisions.Project SummaryThe lab is looking for a highly motivated, proactive and enthusiastic researcher to help build upon the single-cell transcriptomics technology implemented in Maizels et al. The aim will be to develop affordable new sequencing technologies with increased throughput and resolution, facilitating novel research that would previously not be feasible.Firstly, this work will feed into multiple on-going projects within the lab studying the transcriptional dynamics that govern cell fate decisions. Additionally, this work will connect with the projects of multiple collaborators working across clinical, diagnostic and commercial settings, who wish to apply the technology to questions spanning cancer therapeutics, immune profiling, brain disorders and neurodegenerative diseases.The project will involve designing and performing experiments with tissue culture cells and carefully optimising novel genomics protocols. The Laboratory Research Scientist will work in collaboration with other lab members and the ability to work in a team is essential.Key responsibilitiesThese include but are not limited to:

• Working with tissue culture cells
• Designing and performing sequencing and molecular biology experiments
• Operating automation protocols with liquid handling robots
• Record keeping
• Data analysis & interpretation

Key experience and competenciesThe post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:EssentialQualifications, experience and competencies:The candidate should be open-minded, creative, energetic and bring strong expertise and experimental background in at least one of the fields of molecular genetics, embryonic stem cell differentiation systems, or genomics technologies. Basic knowledge of molecular cell biology (e.g. cell culture, RT-qPCR, electrophoresis gels) is essential. Preference will be given to candidates with knowledge and experience in single-cell molecular biology methods and/or lab automation through liquid handling robotics.

• BSc or equivalent in biochemistry or molecular biology or equivalent experience
• Good knowledge and experience in eukaryotic gene regulation, including experience in running PCR reactions and primer design.
• Experience in molecular biology and gene expression assays
• Experience in mammalian cell culture
• Evidence of data presentation in research setting or in publications
• Experience of experimental design, project management and record keeping
• Ability to work independently and capable of collaborating within a group

Desirable

• PhD in molecular and cellular biology
• Experience developing molecular biology and/or sequencing methods
• Experience with mouse embryonic stem cell culture and differentiation systems
• Experience with (or enthusiasm to learn about) single-cell sequencing methods
• Experience with liquid handling robots

Find out what benefits the Crick has to offer:For more information on our great pay and benefits package please click here:Equality, Diversity & Inclusion:We welcome applications from all backgrounds. We are committed to providing equal employment opportunities, regardless of ethnicity, nationality, gender, sexual orientation, gender identity, religion, pregnancy, age, disability, or civil partnership, marital or family status. We particularly welcome applications from people who are Minority Ethnic as they are currently underrepresented in the Crick at this level.Diversity is essential to excellence in scientific endeavour. It increases breadth and perspective, leading to more innovation and creativity. We want the Crick to be a place where everyone feels valued and where diversity is celebrated and seen as part of the foundation for our Institute’s success.The Crick is committed to creating equality of opportunity and promoting diversity and inclusivity. We all share in the responsibility to actively promote dignity, respect, inclusivity and equal treatment and it is our aim to ensure that these principles are reflected and implemented in all strategies, policies and practices.Read more on our website:

Expected salary:

Location: London

Job date: Fri, 20 Sep 2024 04:23:55 GMT

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